Listen In - Bitesize Bio Webinar Audios
Science Podcasts
The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/
Location:
United Kingdom
Genres:
Science Podcasts
Description:
The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/
Language:
English
Episodes
5 Principles for Creating a Rewarding Career Inside or Outside of the Lab
3/23/2023
#64 — Only a tiny percentage of bioscience PhD students will progress to a PI position. This statistic means that ~95% of us need to find a career path other than the traditional academic one most of us aim for when we set out as scientists.
Listen in to hear from Bitesize Bio's founder, Dr. Nick Oswald, and discover five simple but powerful principles you can use to carve out the career that best suits, excites, and rewards you inside or outside of the lab.
You'll discover:
• How to...
Duration:01:00:56
Precise, high-efficiency editing of stem cells to probe human biology and model disease
3/2/2023
#63 — Discover how combining two nobel-prize winning technologies—cell reprogramming and CRISPR gene editing—creates a powerful platform to study basic cell biology, development, and disease. You'll learn how this technology overcomes problems with more traditional immortalized cell models and see how it is already being used to study neurodegenerative diseases.
This webinar is presented by Bill Skarnes, Professor of Cellular Engineering at The Jackson Laboratory.
Follow Bill and The...
Duration:00:49:32
Base editing in practice
2/23/2023
#62 — Base editing allows researchers to make precise changes to the genome. Discover how this technique works, how it differs from traditional genome editing, and how it is being used to understand human variation.
This webinar is presented by Alexis Komor, Assistant Professor at The University of California, San Diego.
Follow Alexis and The University of California, San Diego on Twitter.
Click here to view the full presentation of this webinar.
Browse all episodes of the Listen In...
Duration:01:07:01
CRISPRi as an alternative to CRISPR-cut in human iPSCs
2/16/2023
#61 — Get help and advice with designing and using CRISPRi in human iPSCs. Discover that CRISPRi is an efficient way to silence transcription, sometimes in a complete manner, with loss of H3K4me3 activation marks. Learn how to eliminate side effects of clonal expansion and sites of viral vector integration by using a method that avoids single-cell selection. You'll hear considerations of the challenges of the methods, such as target-specific lentiviral silencing, efficient silencing levels,...
Duration:00:21:31
The 4 D’s of CRISPR gene editing: Design, Develop, Deliver and Detect
2/9/2023
#60 — Whether you are just getting started with CRISPR or want to refresh the basics in designing and performing CRISPR experiments, Dr. Antony Adamson can help. [1] As Manager of the Genome Editing Unit at the University of Manchester, [2] he's helped numerous researchers achieve their varied CRISPR goals. Tap into his knowledge and wisdom in this enlightening presentation; it could just be the advice you need to increase the effectiveness of your CRISPR experiments.
View the full...
Duration:00:58:48
The World of Macrophages: How to Harness Them for Research
9/5/2022
Although macrophages were first described by Elie Metchnikoff in 1882, plenty of mysteries are still associated with the cell type. Indeed, while macrophages were once considered simply “garbage trucks” of the immune system due to their phagocytic property, their substantial and multifaceted contribution to immunological responses and homeostasis is becoming more apparent. Macrophages can produce a wide range of cytokines and chemokines to influence the immune response toward healing or...
Duration:00:59:19
Basics of FLIM and Applications to FRET Biosensors
9/1/2022
Functional imaging is a rapidly growing field key to driving new understanding in biology. Insights into the function and interaction of molecules are the key to reveal the underlying cellular mechanisms. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune to concentration artefacts and sensitive to molecular environment such has pH changes, ion concentrations, and more.
Förster Resonance Energy...
Duration:00:50:05
Adding Multiplex Factor to Confocal Imaging with ZEISS LSM
9/1/2022
In this tutorial on confocal imaging, you will learn how you can:
– Capture weaker signals—and still get sound, reproducible data.
– Reach faster volumetric imaging without sacrificing resolution
– Increase data throughput for your imaging needs
Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes,...
Duration:00:48:09
Cryo-electron Tomography for Cell Biology
9/1/2022
In this webinar, you will learn:
– The complete workflow for in situ cryo-electron tomography
– How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae)
– How mapping these structures back into the native cellular environment reveals new molecular interactions that are only accessible by this technique (e.g., the binding of cargo to COPI-coated Golgi membranes and the tethering of...
Duration:00:58:47
Cryo-Electron Microscopy and the Complexity of Cancer
9/1/2022
In this webinar, you will learn:
– How structural biology can change the face of your research
– The biological challenges of cancer research
– Advantages of cryo-EM for your research
Covering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure.
In single-particle cryo-EM, hundreds of thousands of images formed by electron scattering of...
Duration:00:58:23
Fast superresolution microscopy – with Lattice SIM
9/1/2022
Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and many different labels.
In this webinar, we will explain how Elyra 7 with Lattice SIM takes you beyond the diffraction limit of conventional microscopy to image your samples with superresolution. Learn how to examine the fastest processes in living samples – in...
Duration:00:56:02
Array Tomography for SEM 3D Reconstruction
9/1/2022
Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement.
In this webinar you will get tips and tricks to optimize the workflow of your array tomography:
– Fast and precise trimming of the sample block-face
– Adhesion of a single section to create ribbons
– Automated serial sectioning with the ARTOS 3D ultramicrotome
– Acquisition and...
Duration:00:16:18
Expanding the limits of EM sample prep with ICE
9/1/2022
In this webinar you will learn:
– Improved vitrification: Specimen vitrification without synchronisation fluid
– Vitrification strategies: Optimized freezing for different techniques
– Light and electrical stimulation: Dissect cellular processes with millisecond precision
– A look into the future: freezing of crystals
Plunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery of the cell and how molecules are involved in cellular...
Duration:00:57:31
Using CRISPR/Cas9 to detect DNA sequences with AFM
9/1/2022
Join Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging.
In this webinar, you will learn:
1. How CRISPR/Cas9 can be used to “flag” alterations and mutations in DNA, rather than cut it
2. How pairing atomic force microscopy (AFM) with optical equipment found in DVD players can be used to map DNA at a faster rate than traditional DNA sequencing
3. Applications...
Duration:00:37:41
How to Use CRISPR to Accelerate Cancer Therapies
9/1/2022
Join Theo Roth as he describes his lab’s novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific sites in the genomes of primary human T cells, and permits individual or multiplexed modification of endogenous genes. Importantly, avoiding the use of viral vectors will result in accelerated research and clinical applications, reduce...
Duration:01:10:12
Nucleic acids 101: Confirming Their Quality
9/1/2022
Join us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn:
– How to choose the best method to extract your nucleic acids
– Which method you should chose to determine nucleic acid quality
– How to avoid common pitfalls for both extraction and quality control
Virtually all experiments in the molecular biology lab require high quality, pure nucleic acids as a starting material. This sounds simple enough. However,...
Duration:00:12:18
Live Cell Isolation by Laser Microdissection
9/1/2022
In this webinar, you will learn:
– How to best prepare your specimen for live cell isolation by laser microdissection
– How to optimize your laser microdissection workflow
– How to avoid common pitfalls of this technique
Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike...
Duration:00:51:38
Reliable quantification of fluorescence images
9/1/2022
In this webinar, you will learn:
– How to overcome issues with stage drift and crosstalk between channels
– How to obtain reliable images and reproducible quantification results
– How to proceed with advanced image analysis after restoring images
During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of...
Duration:00:38:33
Crash Course: Developing a Fool-Proof ELISA
9/1/2022
Join us in this webinar featuring Dr. Omonse Talton who will guide you through developing a “fool proof” enzyme-linked immunosorbent assay (ELISA). In this webinar, you will learn:
– When and why you should use the different types of ELISAs– direct, indirect, sandwich, competitive/inhibition ELISA
– Major considerations for developing your ELISA
– Tried and tested tips for you to perform a successful ELISA
The ELISA is one of (if not the most) common techniques in biology and...
Duration:00:48:12
Discovering PARP inhibitor resistance with CRISPR
9/1/2022
In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance to the potent PARP inhibitor talazoparib (BMN 673). The screen yielded one particularly interesting point mutation in the PARP1 gene. This mutation disrupted the ability of PARP1...
Duration:00:47:08