Cannabis Cultivation and Science Podcast

Can stress actually improve flower quality? In this episode, I sit down with Dr. José Leme to dive into the science of plant stimuli and how growers can apply it in practice. We cover: 🌿 Mechanical cues — how super cropping and vibration trigger plant defense pathways. 💧 Water restriction — why moderate, timed drought can shift carbon toward secondary metabolites like terpenes and cannabinoids. 🧪 Jasmonates (MeJA) — when they increase trichome density, and what trade-offs you need to consider. 🔬 Trichome measurement pitfalls — why bracts are the gold standard for consistent data. 🌱 Propagation & trial design — the importance of clonal fidelity and standardized protocols when testing stress strategies. Dr. Leme and I also highlight practical guardrails for growers: cultivar differences, timing, and the “sweet spot” between beneficial stress and damaging stress. 📄 Referenced Studies: Water Deficit Stress and Secondary Metabolites Jasmonates in Growth, Trichomes, and Cannabinoids Metabolic Responses to Water Deficit Trichome Sampling & Quantification Methods Trigonelline as a Biomarker for Drought Stress (Preprint) Propagation & Clonal Fidelity in Cannabis Research Additional vibration study discussed in the episode. 🎧 Whether you’re a grower, consultant, or researcher, this conversation will give you a framework for using stress not as a risk—but as a precision tool to enhance plant quality.

This week on the Cannabis Cultivation and Science Podcast, we're diving deep into lighting and photobiology with Shane Torpey, founder of MIGRO. With a background in engineering and construction, Shane turned his passion for growing into a global grow lighting company known for trusted reviews and educational content. We break down what’s working—and what’s not—in cannabis lighting for 2025, including: Far-red and intra-canopy lighting LED vs. HPS and how to transition Maximizing PPFD without added CO₂ Dimming schedules at sunrise/sunset The impact of lenses on LED light penetration What really matters when choosing a grow light Emerging technologies shaping the future of cultivation Whether you’re scaling a facility or dialing in your home grow, this episode gives you the tools to make smarter lighting decisions rooted in science and real-world data. Links to channels here: https://www.youtube.com/@MIGROLIGHT https://www.instagram.com/migrolight/ https://www.facebook.com/MIGROLIGHT/ https://www.tiktok.com/@migrolight

📝 Episode Highlights: Connor’s journey from greenhouse grower to propagation leader Scaling propagation: key infrastructure and protocols Hormonal treatments and substrate selection for optimal rooting Managing cultivar libraries and mother plant health IPM and environmental control strategies tailored for veg Writing and refining SOPs for compliance and consistency Track-and-trace integration in a regulated cannabis market The role of living soil vs. hydroponics in Connor’s career 🧪 Mentioned in This Episode: Collective Elevation: www.collectiveelevation.com KIS Organics Products & Podcast: www.kisorganics.com Cannabis Cultivation & Science Conference: September 5–7, 2025, Bozeman, Montana — featuring Suzanne Wainwright-Evans, Dr. Greenhouse, Jeremy Plumb, and more. Early bird tickets available now! Join our Patreon for bonus content: Patreon.com/KISOrganics

In this episode of the Cannabis Cultivation and Science Podcast, we’re joined by Dr. Michael Gutensohn, an expert in plant physiology and secondary metabolism, to explore the intricate relationship between environmental stress and the production of secondary metabolites in cannabis. We discuss how various abiotic stressors—such as light intensity, temperature fluctuations, and nutrient availability—can influence the biosynthesis of key phytochemicals, including cannabinoids, terpenes, and flavonoids. Dr. Gutensohn shares insights from his research on plant defense mechanisms, metabolic signaling pathways, and how growers might harness controlled stress to enhance both plant resilience and phytochemical output. Whether you’re a cultivator aiming to fine-tune your grow environment or a scientist delving into cannabis metabolomics, this episode offers valuable, research-backed perspectives on optimizing secondary metabolite production through stress modulation.

Welcome to the Cannabis Cultivation and Science podcast, I'm your host Tad Hussey of KIS Organics. This is the podcast where we discuss the cutting edge of growing from a science based perspective and draw on top experts from around the industry to share their wisdom and knowledge. My guest this week is Bryant Mason. I’ve had Bryant on the podcast a few times in the past and he’s one of my favorite guests. Bryant is a Certified Crop Advisor who specializes in organic cannabis nutrition. He has spent the last 8 years working to combine precision laboratory techniques with data science in an effort to unravel the complexities of cannabis soil and crop nutrition. Along with his work on soilless media, he also helps outdoor cultivators around the U.S. with nutrient management and cover cropping. If you would like to do soil testing, help interpreting a soil test, or are interested in an online course on these subjects, his website is www.soildoctorconsulting.com. If you want to save $100 on his course, you can use the discount code KISORGANICS, all one word. I’ve taken his course and was recently going back to review some of the content. It’s excellent and I highly recommend it for anyone wanting to do a deep dive on cannabis fertility and re-using your soil. After recording the podcast with Bryant, he had a couple of points he wanted to clarify so there is a short addendum that I added in at the very beginning of the podcast. Now on to the show!

Before starting the podcast, I wanted to share about an exciting new conference that we are hosting in conjunction with Collective Elevation in Bozeman, Montana. I’ve been talking to Suzanne Wainwright Evans about hosting a conference for years, and Adam at Collective graciously offered to open up his doors and let us host it on his property. The event is happening on September 5-7th, 2025 and I’m super excited. We are kicking it off with workshops from The Bug Lady Suzanne and Dr. Greenhouse, followed by a facility tour and then a bunch of excellent speakers and networking events over the next 2 days. Then on the 7th, the Montana Living Soil Alliance is hosting a tasting event that will be included in the price of the conference ticket. The tasting event will be a private event, with cannabis donated by the Living Soil Alliance, as a way to have an amazing sensory experience and see what premium quality Montana Living Soil has to offer. Tickets are limited to 200 spots, so be sure to grab a ticket if you’re interested. Tickets are now on sale at kisorganics.com and the conference is called the Cannabis Cultivation and Science Conference. In honor of the conference, I wanted to do an interview series with some of the amazing folks at Collective Elevation to give a growers perspective and share some of what they’ve been able to accomplish. I’ve already had Adam Arnold on the podcast and you can also view a tour of their facility on the kisorganics youtube page. My guest this week is Jared Ferguson, the Greenhouse Manager at Collective Elevation. He’s been working at Collective for over 4 years now, but prior to that he studied Environmental horticulture at Montama State University in bozeman. During which he worked for the universities greenhouse facility and research farm, where hje got his first experiences in mixing soil, amending plants, managing irrigation, and conducting IPM processes. Now on to the show!

Saul began his agriculture work in vineyard and winery operations on the Central California coast. After deciding to focus his career on crop protection he moved north where he managed IPM programs at Monterey Mushroom Company and Jacobs Farm/Del Cabo. This work led to a position as IPM Manager at Harborside Farms where he directed their bio-intensive greenhouse cannabis IPM program. Saul now works as a Technical Sales Representative for BioBee USA.

Dr. Nadia Sabeh is the President and Founder of Dr. Greenhouse, Inc., an agricultural and mechanical engineering firm that specializes in the design of HVAC systems for indoor plant environments. Dr. Sabeh has her PhD in Agricultural Engineering from the University of Arizona’s Controlled Environment Agriculture Center (CEAC) and is a licensed Mechanical Engineer in the State of California. For over 20 years, she has dedicated her education and career to helping farmers control their environments, allowing them to grow crops indoors, in greenhouses and in facilities that would otherwise be impossible or impractical to do so. Nadia has a free VPD app for both iPhone and Android as well as an amazing podcast called "The Doctor is in" that's available on all streaming platforms. https://www.doctorgreenhouse.com/podcast/tag/podcast

My guest this week is my good friend, Suzanne Wainwright-Evans also known by many as The Bug Lady. She is an Ornamental Entomologist specializing in integrated pest management. Suzanne has been involved in the Green Industry for more than 25 years with a primary focus on biological control and using pesticides properly. She is a graduate of the University of Florida with degrees in both Entomology and Environmental Horticulture. Suzanne has been on the podcast multiple times and I highly suggest folks go back and listen to some of her other podcasts if you haven’t already. Her website is bugladyconsulting.com where you can find links to other webinars, podcasts and information as well as how to contact her for direct consulting. Now on to the show!

My guest this week is my good friend, Suzanne Wainwright-Evans also known by many as The Bug Lady. She is an Ornamental Entomologist specializing in integrated pest management. Suzanne has been involved in the Green Industry for more than 25 years with a primary focus on biological control and using pesticides properly. She is a graduate of the University of Florida with degrees in both Entomology and Environmental Horticulture. Suzanne has been on the podcast multiple times and I highly suggest folks go back and listen to some of her other podcasts if you haven’t already. Her website is bugladyconsulting.com where you can find links to other webinars, podcasts and information as well as how to contact her for direct consulting. Now on to the show!

This was a fun podcast for me because I think there's a lot of misinformation around this topic and it was great to hear from an expert! Sphagnum Peat Moss is the gold standard for the horticulture industry. In N. America we use the majority of our peat for growing plants. Are there better options? What's the current research tell us about the sustainability of peat moss? In this episode we talk about why peat moss is such a popular choice for media as well as the pros and cons of the current substrates on the market.

My guest this week is Christine DeJesus. Christine operates at the intersection of regenerative organic agriculture and social justice. For five years she’s been perfecting a highly efficient organic cultivation model while managing operations for Galenas Cannabis in Akron, Ohio. Prior she worked in food justice, ran a farm for Great Lakes Brewing Company, functioned as campaign coordinator for Marijuana Policy Project, and co-founded the Midwest CannaWomen. She was featured in the documentary “Polycultures: Food Where We Live,” with Michael Pollan, and at the “My Global Kitchen” exhibit at the Cleveland Museum of Natural History. Christine is a creative writer, a columnist for Cannabis Business Times magazine, and holds degrees from both Kent State and the University of Pittsburgh. In her current role, she focuses on keeping plants, processes, and people healthy and productive while delivering top-tier quality cannabis flower to Ohio consumers. Christine also works with home-growers to bring commercial-level success to their backyard gardens.

Christine Goedhart is an experienced educator and educational developer with a specialization in teaching undergraduate biology courses. Currently serving as a Science Education Specialist at the University of British Columbia, she supports faculty in making their courses more equitable and student-centered, and she recently developed and taught an undergraduate Biology of Cannabis course. Previously, Christine spent eight years as a full-time faculty member at Citrus College, where she taught a variety of biology courses for non-major students. With a strong commitment to student-centered and inclusive education, Christine has effectively implemented innovative pedagogical and assessment strategies to enhance student engagement and learning in biology courses, and she shares information, tips, and evidence-based best practices for teaching in her Tips for Teaching blog article series.

My guest this week is Matthew DeBacco. Matthew received his Bachelors degree in Pathology with a secondary degree in horticulture, then going on to receive a master’s degree in agronomy 2011 and a masters in education in 2014, both from the university of Connecticut. Then in 2020 he received his PhD from Ashford University. Matthew started the first cannabis course at a public university at the University of Connecticut. I first found out about Matthew from his research on powdery mildew with cucurbits using raw milk, as it was circulating around the giant pumpkin community. Matthew also enjoys growing giant pumpkins and has held the state record twice with the largest weighing 1,885.5 lbs. In this episode we talk about the similarities between the giant pumpkin community and the cannabis community and how we might be able to utilize some of the advancements across the cultivation of these very different plants. Now on to the show!

My guest this week is Dr. Adel Zarei. Dr. Zarei earned his Ph.D. degree from Leiden University in the Netherlands, specializing in molecular mechanisms governing defense and secondary metabolism in plants. Dr. Zarei spent a decade as a Research Associate at the University of Guelph, where his research focused on stress physiology in plants. His primary passion lies in the study of plant secondary metabolites, many of which possess pharmaceutical significance. In 2017, Adel transitioned to the Cannabis industry, serving as a Scientific Advisor and Cannabis Micropropagation Expert. He played a pivotal role in designing and establishing multiple Cannabis Micropropagation labs for Cannabis Production License Holders. His groundbreaking work in this field garnered multiple Government grants and led to the development of commercial-scale cannabis micropropagation techniques. The results of this research were published in prestigious peer-reviewed journals, including In Vitro Cellular and Developmental Biology-Plant and Plants”. Throughout his scientific career, Adel has authored more than twenty-two peer-reviewed articles covering topics such as plant stress physiology, secondary metabolites, and plant pathology. Dr. Zarei is an integral member of the PharmaCrop team, serving as the Chief Operating Officer at PharmaCrop Pty. Ltd. in Queensland, Australia. In this role, he oversees operations that encompass every stage of the process, from seed cultivation to the final production of medical-grade cannabis products. Dr. Zarei has successfully transferred his extensive knowledge and experience in the cultivation and processing of medical cannabis into Australia burgeoning industry. Now on to the show!

My guest this week is Adam Arnold is a founder/ Owner at Collective Elevation in Bozeman Montana. Collective Elevation is one of the largest living soil cannabis greenhouse facilities in the country. Adams been growing cannabis for 20 years, the last 10 at his facilitiy in Bozeman which was designed and built from the ground up by him and his team. Prior to recreational legalization in Montana he built off grid greenhouse facilities in Montana during the medical days and has founded Collective Elevation on a platform of sustainability utilizing sun assisted living soil practices. He went to school at the university of Idaho for Conservation Biology and Natural Resource Ecology and worked in wilderness management for the Forest Service prior to working in the Cannabis industry. I had the pleasure of visiting Collective Elevation last year and filmed my tour, which is available on Youtube, I highly suggest checking it out. It’s one of the nicest facilities I’ve ever seen in person and I’m honored that they have been using our soils in their greenhouses for a few years now. Anyway, now on to the show!

Dr. Tassa Saldi is an accomplished molecular biologist with more than 25 years of research experience in RNA biology, infectious disease, and molecular pathogen detection. Dr. Saldi graduated Summa Cum Laude with a degree in Molecular Biology. She completed her graduate and postdoctoral studies at the University of Colorado, where she studied RNA structure, small RNAs and RNA processing. She was awarded a prestigious fellowship from the American Cancer Society and was one of eighteen doctoral fellows nationwide invited to present her research at the Aspen Cancer Conference. Dr. Saldi has authored more than 20 peer-reviewed articles in top-tier journals. As the Chief Science Officer and co-founder of TUMI Genomics, Dr. Saldi continues to use her expertise to provide the cannabis industry with reliable, accurate diagnostic tools and pathogen mitigation guidance.

My guest this week is a returning guest. Dr. Nicole Gauthier is a pathologist, and Professor and Extension specialist at the University of Kentucky. Her program focuses on disease management of specialty crops, including hemp. In her Extension role, she develops educational and outreach programs to help growers manage disease through identification, understanding of pathosystems, and integration of management strategies. Her research program focuses on a range of hemp diseases, including Fusarium head blight. Dr. Gauthier earned her BS in Horticulture Science and her PhD in Plant Pathology from Louisiana State University. She joined the UK College of Agriculture in 2011 and began working with industrial hemp in 2014. There will be a link on the podcast page to access her resources and research. Now on to the show! Hemp and cannabis research and Extension fact sheets https://plantpathology.ca.uky.edu/extension/publications#HEMP Silicon study https://plantpathology.ca.uky.edu/files/pprr-02.pdf

George has degrees in Horticulture from the University of Georgia and the University of Florida. He is the GGSPro Technical Services Manager with Griffin Greenhouse Supplies. GGSPro assists ornamental, edible, and hemp/cannabis growers across the country with their cultivation issues ranging from sanitation, IPM, fertility, lighting, and water quality. GGSPro fields over 17,000 grower related calls and emails annually. This amount of valuable interaction helps Griffin collaborate with chemical vendors, academic researchers, and plant producers to implement change within the green industry.

My guest this week is June Graesch from Bioworks. Julie has 18 years of experience as a biological scientist, practiced in laboratory, greenhouse, and field research as well as technical service support for the integrated pest management industry. She has been with BioWorks for 5 years and has previously held positions at BASF, Becker Underwood, and Iowa State University. Now on to the show!